RNA-seq of Ricotia lunaria: floral development stage - SRA

SRX4225392: RNA-seq of Ricotia lunaria: floral development stage
1 ILLUMINA (Illumina HiSeq 2000) run: 39.9M spots, 8.1G bases, 5Gb downloads

Design: Total RNA samples were pooled together with the root, stem, leaf and bud with the ratio of 1:1:1:2. The poly-A mRNA was enriched with magnetic Oligo (dT) beads and then fragmented into short fragments as the templates to synthesize the cDNA fragments. The cDNA fragments were further purified with a QiaQuick PCR extraction kit (QIAGEN Inc., Valencia, CA, USA), followed by end repairing and tailing A, and then were ligated to sequencing adaptors. The required length ligation products with 200-700 bps were purified by agarose gel electrophoresis and enriched by PCR amplification. Finally, the paired-end library was sequenced by the Illumina HiSeqTM 2000 platform with the average read length of 90 bp.

Submitted by: Cold and Arid Regions Environmental and Engineering Research Institute

Study: two ecotypes of Ricotia lunaria Raw sequence reads

PRJNA476478 • SRP150700 • All experiments • All runs

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transcriptomes of two ecotypes of Ricotia lunaria, which habitated on the opposite slopes of EC I (Lower Nahal Oren, Mt. Carmel, Israel) with only 100 meters apart from each other

Sample: Plant sample from Ricotia lunaria

SAMN09435491 • SRS3424971 • All experiments • All runs

Organism: Ricotia lunaria

Library:

Name: NFS5_T1

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 39.9M spots, 8.1G bases, 5Gb

Run	# of Spots	# of Bases	Size	Published
SRR7352168	39,903,639	8.1G	5Gb	2018-11-15

ID:: 5712398

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